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PGT TGT Biotechnology Syllabus, Exam Pattern

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📅 Updated On 22 Oct, 2024✨ Update By Divya Mehra🌐 Hindi & English

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Combo PackagePGT TGT Biotechnology

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Combo - 2 (English)

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Combo PackagePGT TGT Biotechnology

PGT TGT Biotechnology

Combo - 3 (English)

1. Online Mock Test Series in English = 17 Tests.
2. Question Bank - 1700 Questions with Answer.
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Combo PackagePGT TGT Biotechnology

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Combo - 4 (English)

1. Online Mock Test Series in English = 17 Tests.
2. Question Bank - 1700 Questions with Answer.
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PGT TGT Biotechnology Syllabus, Exam Pattern

PGT TGT Biotechnology Syllabus, Exam Pattern

PGT TGT Biotechnology Syllabus
 
Biotechnology : -
 
1. Introduction to Bio-technology: Historical perspectives, scope and importance, commercial potential , interdisciplinary challenge, a quantative approach-scale up – stages in commercialization of product and process, the fermenter , aseptic operation. Manufacturing quality control, good manufacturing practices, good laboratory practices, product safety, bio safety principles-environment and health risk , assessment, bio safety regulatory guidelines and controlling agency, environmental law for hazardous drugs , microbes and GMO’S, Biotechnology related issues of Public concern, Bioethics. Marketing, Biotechnology in India
and global trends.
 
2. Fundamentals of Biochemical engineering- Concept of pH, buffer, physical variables, dimensions and units, measurement conventions, physical and chemical properties, data, stoichiometry, errors in data and calculation, absolute and relative un certainty and types of error statistical analysis presentation of experimental data, data analysis, trends, testing mathematical models, goodness to fit, use of graph paper with logarithmic coordination and plotting of data process flow diagrams, material balance, fluid flow and mixing, mass transfer, heat transfer, unit operations, homogenous reactions, microbial growth, substrates utilization and product formation kinetics, reactor engineering – rheology of fermentation fluids, scale up concepts, design of fermenting media, aseptic transfer, various microbial and enzyme reactors, instrumentation in bio reactors.
 
3. Biotechnology and Society- Public perception of Biotechnology intellectual property, patents, reading a patent, International scenario, National scenario, Varietals protection, ethical issues in agriculture and health care. 
 
4. Biochemistry: Biomolecules- Structure and Dynamics; Thermodynamics: concept of free energy, entropyBuilding blocks of carbohydrates – sugars and their derivatives , chemical properties of sugar, polysaccharides – glycogen, cellulose, chitin etc. Building blocks of proteins – Amino acids, Chemical properties of amino acids, regulation of amino acid metabolism and inborn errors of metabolism determination of sequencing of amnino acids, fragmentation of polypeptide chain, 3D
structure of proteins, secondary, tertiary and quarternary structure of proteins, vitamins and enzymes. Lipids –simple fatty acids, Sphingosine, Glycerol and cholesterol and their chemical properties, lipid metabolism and its regulation. Nucleic acids- Nucleo tides, chemical properties, optical activities and stereo chemistry of bio molecules, polarimetry, conformations and configuration, RNA, DNA, 3D model of DNA, chromosome structure, circular and super coiled DNA. Biochemical transformations-carbohydrates metabolism-glycolytic path way, krebs cycle, homo fermentative path way. KH, PPP, photosynthesis- light reaction Kelvin cycle, nitrogen fixtation, nitrogen cycle, nitrogenase , gluconeogenesis, electron transport and oxidative phosphorylation, precursor-product relationship, supramolecular assembly, biomolecular database, biomembranes, structure and function of liposomes and their applications
 
5. Techniques, Instrumentation and principles :
 
Techniques based on molecular weight or size- Centrifugation and ultra centrifugation, gel permeation, osmotic potential.Techniques based on polarity- Ion exchange chromatography, elctrophoresis, isoelectric focusing , hydro phobic interaction , partition chromatography.Techniques based on spectroscopy- Colorimetry, UV visible , spectro photometry, fluorescence , spectroscopy , x-ray crystallography , mass spectrometry, radio isotopes techniques; Techniques based on solubility – Salt perceipitation, precipitation with organic solvent.
 
Cellular techniques- Microscopy-LM,TEM,SEM cell sorting , cell fractionation , cell growth determination , electronic particle counter, culture based counting methods Genetical
 
techniques- Chromosomal techniques- Staining , bending, pattern, Karyotyping, chromosomal painting. Mutagenic techniques- Bacterial and seed mutagenesis , recombination in bacteria, conjugation, transduction, breeding methods in plants, pedigree analysis, DNA isolation.
 
6. Cell Biology: Cell structure and components- Cell membrane – composition, Structure, membrane , associated receptors, artificial membrane, membrane proteins, principals of membrane organization, cell junction, membrane lipids. Cell organellels – Golgi bodies, Endoplasmic reticulum, lysosomes, per oxisomes, ribosomes, internatilsation of macro molecules , endo and exocytosis , mitochondrial structure and oxidative phosphorylation. Cytoskeleton- Micro tubules , micro filaments, lattice and cytosol ;Nucleus –nuclear envelope , nucleolus , chromosome tissue and organs , evolution and population , speciation , biodiversity ,
adaptation, natural selection, organization of life, size and complexity , interaction with environment .Cell growth and development - Cell division, cell cycles, cell communication and signal transduction , movement, nutrition, gaseous exchange , internal transport, maintaining the internal environment, reproduction, animal and plant development, immune response, apoposis, plant-pathogen relation, secondary metabolism, defence strategy in microbes and insects.
 
7. Genetics and Molecular Biology :
 
Principles of Genetics- Mendelian genetics, role of chromosome in inheritance, multiple alleles, linkage and crossing over, genetic recombination, genetic mapping, gene interaction, sexed linked inheritance, extra nuclear inheritance, quantitative inheritance, genes at the population level, discovery of DNA as genetic material-Griffiths experiment, Hershey and Chase experiment, mutagenesis , types of mutations, genome, chromosome and gene mutations, molecular mechanism of mutation, DNA repair, genetic disorder, transposons, animal and plant breeding. Genome function- Genome organization, sequencing DNA replication, fine structure of gene, from gene to protein, transcription, genetic code, translation, regulation of gene expression, genetic basis of development, genetic of cancer,
immuno genetics, evolutionary genetics. 
 
8. Protein and gene manipulation :
 
Protein Structure and engineering.- 3D shape of proteins, non covalent bonds, hydrogen bonds, van der waals forces, hydro phobic interaction. Structure function relationships in proteins – Chymotrypsin, molecular disease. protein finger printing, 2D gel electrophoresis, purification of proteins, characterization of proteins, proteins based products, mass spectrometry, blood products and vaccine, therapeutic antibodies and enzymes, hormones and growth factor, regulatory factor, analytical application, industrial enzymes , functional non catalytic proteins, nutraceutical proteins, designing proteins, proteomics, genes and proteins type of proteomics .
 
Recombinant DNA Technology - Tools of recombinant DNA technology, restriction enzymes, making of recombinant DNA , DNA library, introduction of recombinant DNA into host cells-plasmid , cosmid, vectors, lambda, bacteriophage , identification of recombinants , PCR, DNA probes, hybridization techniques , DNA sequencing, site directed mutagenesis, cloning strategies.
 
Genomics and Bioinformatics- Structural and functional genomics, genome sequencing projects, genetic mapping, gene prediction and counting , genome similarity, SNPs and comapartive genomics , functional genomics-micro array techniques, fluorescence, in situ hybridization , comparative DNA hybridisation, history of bio informatics, sequences and nomenclature, DNA and protein sequences, information sources-major databases, blast family search tools, resources for gene level sequences, analysis using bio informatics tools.
 
9. Cell culture technology :
 
Microbial cell culture and its applications- nutrients, energy sources, sterilization procedures, environment for microbial growth, aeration and mixing , equipments for culturebioreactors, Types of microbial culture, measurement and kinetics of microbial growth, scale up of microbial process, isolation of microbial products, strain isolation and improvement, application of microbial culture technology bioethics.
 
Plant Cell culture and applications- Cell and tissue culture techniques- Nutrient media, types of cultures, plant regeneration pathways, application of cell and tissue culture, gene transfer methods in plants, transgenic plants with beneficial traits, stress tolerance, herbicide tolerance, insect resistance, transgenic plant as bio reactor, diagnostics in agriculture and molecular breeding, morphological and molecular markers , bioethics.
 
Animal cell culture and applications- Primary cell culture, secondary cell culture and lines, types of cell lines, physical environment, osmolality, media, pH temperature cryopreservation, equipments required for animal cell culture, carbon dioxide incubators, Characterisation of cell lines- Scale up of animal culture, applicantions of animal cell cultureTissue plasminogen activator, factor VIII, erythropoietin, hybridoma technology, mono colonal antibodies, therapeutic antibodies, stem cell technology- morphological approach, in vitro clonal assay, long term marrow culture, embryonic stem culture, cell and tissue engineering, bioethics in animal genetic engineering. 
 
10. Immunology :
 
Immune system, molecules of immune system, immuno globulins, MHCs, cytokines, T cell receptor, generation of antibodies and T cell receptor diversity, complement system, humoral and cell mediated immunity, immune regulation, vaccines, hybridoma, immuno deficiencies, AIDS, transplantation immunity and cancer.
 
11. Applied Biotechnology :
 
Biotechnology industry , Bioinformatics, molecular technology for diagnosis of genetic disorders, onco viruses and immunity, lymphocyte, homoestasis, viral induced modulation of host immune response, HLA polymorphism, induction and maturation of B cells, safe limits for radiation determined , radiation carcinogenesis.
 
PGT TGT Biotechnology Exam Pattern
 
Duration : 60 Minutes
 
No.of Question : 100
 
Total Marks : 100
FAQs

PGT TGT Biotechnology Exam Syllabus Frequently Asked Questions (FAQ's)

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In this article Page, we have provided the required syllabus of the PGT TGT Biotechnology exam.
Being familiar with PGT TGT Biotechnology Exam Pattern will help you understand the types of questions asked, difficulty level of the exam and important topics that require your keen attention. It will also acquaint you with the marking scheme and time allotted to each Topics.
PGT TGT Biotechnology syllabus pdf download option is available here which will help you to get the PDF saved in your device that you can access anytime. It consists of a complete syllabus of PGT TGT Biotechnology which you can refer to while preparing for the exams.
PGT TGT Biotechnology exam is not tough but it depends on your preparation. The candidates find the Subject not challenging. The dedicated preparation in the right direction will undoubtedly take you towards the goal.
The candidates must be well versed and acquainted with the syllabus and the exam pattern. Select the areas where improvement is required and schedule your preparation accordingly. To ace this exam, the applicants ought to maintain speed with high accuracy. Practicing previous years question papers will help you understand the exam pattern and the level of difficulty of the exam. You need to hone your strengths and improve upon your weaknesses. Toppersexam.com will aid you through the preparation and drive you to success. At Toppersexam.com, you can practice a complete set of test series along with a free PGT TGT Biotechnology mock test designed by our expert faculty.
The syllabus of PGT TGT Biotechnology Exam includes all topics that are important for the exam and are necessary for thorough preparation. Toppersexam provides a complete and updated syllabus with a detailed topic-wise breakdown.
You can download the complete PGT TGT Biotechnology Exam syllabus PDF from toppersexam.com. The PDF is available free and includes a detailed list of topics, section-wise subjects, and weightage for better exam preparation.
Yes, PGT TGT Biotechnology Exam has a topic-wise syllabus that includes all relevant subtopics. Toppersexam provides the detailed breakdown, helping candidates focus on high-weightage areas and avoid missing important sections.
Toppersexam ensures that the PGT TGT Biotechnology syllabus is updated for 2026. The latest syllabus reflects current exam trends, question patterns, and updated topics as per official notifications.
The PGT TGT Biotechnology Exam pattern includes details like the number of sections, total questions, marks distribution, and exam duration. Toppersexam provides a comprehensive guide on exam format, marking scheme, and question types.
Yes, the majority of questions in PGT TGT Biotechnology Exam are MCQs. Toppersexam provides practice MCQs with answers to help candidates understand the question pattern and improve accuracy.
Yes, questions are divided section-wise. Toppersexam provides a clear guide on how many questions are asked per section and their difficulty level for effective preparation.
Yes, Toppersexam offers online mock tests that strictly follow the PGT TGT Biotechnology Exam pattern. These mocks help candidates practice time management and get real-exam experience.
Yes, exam patterns can change over time. Toppersexam provides updated information on the latest exam pattern, question types, and section-wise changes.
Understanding the exam pattern is crucial for time management, question prioritization, and strategy planning. Toppersexam guides candidates on how to use the pattern effectively to maximize scores.
Start by dividing the syllabus into sections and prioritizing high-weightage topics. Toppersexam provides a preparation strategy, along with topic-wise tips and sample questions to make syllabus analysis easier.
Yes, focusing on high-weightage topics improves efficiency. Toppersexam highlights important topics, helping candidates prioritize sections for better performance.
Toppersexam provides a free downloadable PDF that includes both the syllabus and detailed exam pattern. This helps candidates study offline and revise efficiently.