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UPPSC Assistant Professor (Botany) Syllabus 2026, Exam Pattern, Exam Date 2026
Detail Information about UPPSC has published notification 2026 for the recruitment of Assistant Professor (Botany) vacancies. Those Candidates who are Interested to the following vacancy and completed all Eligibility Criteria can read the Notification & Apply Online. In this page we provide the Complete Syllabus of this Recruitment with Latest Update Exam Pattern and the Exam Date also.
UPPSC Assistant Professor Syllabus 2026 - Overview
UPPSC Assistant Professor (Botany) Syllabus 2026
General Studies :
1. General Science.
2. Current Events of National and International Importance.
3. History of India (Including Indian National Movement).
4. Indian Polity and Economy.
5. Geography- Indian and world.
6. Mental ability and Statistical data analysis.
Candidates are expected to have general awareness about the above topics with special reference to Uttar Pradesh.
Optional Subject (Botany) :
UNIT I: MOLECULES AND THEIR INTERACTION RELEVANT TO BIOLOGY
A. Structure of atoms, molecules and chemical bonds.
B. Composition, structure and function of biomolecules (carbohydrates, lipids, proteins, nucleic acids and vitrains).
C. Stabilizing interactions (Van der Waals, electrostatic, hydrogen bonding, hydrophobic interaction, etc.).
D. Principles of biophysical chemistry (pH, buffer, reaction kinetics, thermodynamics, colligative properties).
E. Bioenergetics, glycolysis, oxidative phosphorylation, coupled reaction, group transfer, biological energy transducers.
F. Principles of catalysis, enzymes and enzyme kinetics, enzyme regulation, mechanism of enzyme catalysis, isozymes
G. Conformation of proteins (Ramachandran plot, secondary structure, domains, and folds).
H. Conformation of nucleic acids (helix (A, B, Z), t-RNA, micro-RNA).
I. Stability of proteins and nucleic acids.
J. Metabolism of carbohydrates, lipids, amino acids nucleotides and vitamins.
UNIT II: CELLULAR ORGANIZATION
A. Membrane structure and function motif (Structure of model membrane, lipid bilayer and membrane protein diffusion, osmosis, ion channels, active transport, membrane pumps, mechanism of sorting and regulation of intracellular transport, electrical properties of membranes).
B. Structural organization and function of intracellular organelles (Cell wall, nucleus, mitochondria, Golgi bodies, lysosomes, endoplasmic reticulum, peroxisomes, plastids, vacuoles, chloroplast, structure and function of cytoskeleton and its role in motility).
C. Organization of genes and chromosomes (Operon, unique and repetitive DNA, interrupted genes, gene families, structure of chromatin and chromosomes, heterochromatin, euchromatin, transposons).
D. Cell division and cell cycle (Mitosis and meiosis, their regulation, steps in cell cycle, regulation and control of cell cycle).
E Microbial Physiology (Growth yield and characteristics strategies of cell division, stress response)
UNIT III: FUNDAMENTAL PROCESSES
A. DNA replication, repair and recombination (Unit of replication, enzymes involved, replication origin and replication fork, fidelity of replication, extrachromosomal replicons, DNA damage and repair mechanisms, homologous and site-specific recombination).
B. RNA synthesis and processing (transcription factors and machinery, formation of initiation complex, transcription activator and repressor, RNA polymerases capping, elongation, and termination, RNA processing, RNA editing, splicing, and polyadenylation, structure and function of different types of RNA, RNA transport).
C. Protein synthesis and processing (Ribosome, formation of initiation complex, initiation factors and their regulation, elongation and elongation factors, termination, genetic code, aminoacylation of tRNA, tRNA-identity, aminoacyl tRNA synthetase, and translational proof-reading, translational inhibitors, post-translational modification of proteins).
D. Control of gene expression at transcription and translation level (regulating the expression of phages, viruses, prokaryotic and eukaryotic genes, role of chromatin in gene expression and gene silencing).
UNIT IV: CELL COMMUNICATION AND CELL SIGNALING
A. Host parasite interaction- Recognition and entry w processes of different pathogens like bacteria, viruses into re plant host cells, alteration of host cell behavior by C pathogens, virus-induced cell transformation, pathogen-po induced diseases in plants, cell-cell fusion in both normal st and abnormal cells.
B. Cell signaling Hormones and their receptors, cell set surface receptor, signaling through G-protein coupled D receptors, signal transduction pathways, second co messengers, regulation of signaling pathways, bacterial E. and plant two component systems, light signaling in plants, st bacterial chemotaxis and quorum sensing.
C. Cellular communication - Regulation of hematopoiesis, F. general principles of cell communication, cell adhesion and in roles of different adhesion molecules, gap junctions, G. extracellular matrix, integrins, neurotransmission and its fu regulation.
UNIT V: PLANT PHYSIOLOGY
A. Photosynthesis Light harvesting complexes mechanisms of electron transport; photoprotective mechanisms; CO, fixation-C3, C4 and CAM pathways.
B. Respiration and photorespiration - Citric acid cycle; plant mitochondrial electron transport and ATP synthesis-alternate oxidase; photorespiratory pathway.
C. Nitrogen metabolism Nitrate and ammonium assimilation; amino acid biosynthesis.
D. Plant hormones - Biosynthesis, storage, breakdown and transport; physiological effects and mechanisms of action.
E. Sensory photobiology - Structure, function and mechanisms of action of phytochromes, cryptochromes and phytotropins; stomatal movement; photoperiodism and biological clocks.
F. Solute transport and photo assimilate translocation uptake, transport and translocation of water, ions, solutes and macromolecules from soil, through cells, across membranes, through xylem and phloem; transpiration; mechanisms of loading and unloading of photo assimilates.
G. Secondary metabolites Biosynthesis of terpenes, phenols and nitrogenous compounds and their roles.
H. Stress physiology Responses of plants to biotic (pathogen and insects) and abiotic (water, temperature and salt) stresses.
UNIT VI: INHERITANCE BIOLOGY
A. Mendelian principles: Dominance, segregation, independent assortment.
B. Concept of gene: Allele, multiple alleles, pseudo allele, complementation tests
C. Extensions of Mendelian principles: Codominance, incomplete dominance, gene interactions, pleiotropy, genomic imprinting, penetrance and expressivity, phenocopy, linkage and crossing over, sex linkage, sex limited and sex influenced characters.
D. Gene mapping methods: Linkage maps, tetrad analysis, mapping with molecular markers, mapping by using somatic cell hybrids, development of mapping population in plants
E. Extra chromosomal inheritance: Inheritance of Mitochondrial and chloroplast genes, maternal inheritance.
F. Microbial genetics: Methods of genetic transfers -transformation, conjugation, transduction and sex-duction, mapping genes by interrupted mating, fine structure analysis of genes.
G. Quantitative genetics: Polygenic inheritance, heritability and its measurements, QTL mapping
H. Mutation: Types, causes and detection, mutant types -lethal, conditional, biochemical, loss of function, gain of function, germinal verses somatic mutants, insertional mutagenesis
I. Structural and numerical alterations of chromosomes: Deletion, duplication, inversion, translocation, ploidy and their genetic implications.
J. Recombination: Homologous and non-homologous recombination including transposition.
UNIT VII: DIVERSITY OF LIFE FORMS
A. Principles & methods of taxonomy: Concepts of species and hierarchical taxa, biological nomenclature, classical & quantitative methods of taxonomy of plants, animals and microorganisms. Levels of structural organization: Unicellular, colonial and multicellular forms.
B. Levels of organization of tissues, organs & systems. Comparative anatomy, adaptive radiation, adaptive modifications.
C. Outline classification of plants & microorganisms: Important criteria used for classification in each taxon. Classification of plants, animals and microorganisms.
D. Natural history of Indian subcontinent: Major habitat types of the subcontinent, geographic origins and migrations of species.
E. Organisms of health & agricultural importance: Common parasites and pathogens of crops.
F. Organisms of conservation concern: Rare, endangered species. Conservation strategies.
G. Biology and Biodiversity of Viruses, Bacteria, Fungi, Bryophytes, Pteridophytes, Gymnosperms and Angiosperms.
UNIT VIII: ECOLOGICAL PRINCIPLES
A. The Environment: Physical environment; biotic environment; biotic and abiotic interactions.
B. Habitat and Niche: Concept of habitat and niche; niche width and overlap; fundamental and realized niche; resource partitioning; character displacement.
C. Population Ecology: Characteristics of a population; population growth curves; population regulation; life history strategies (rand K selection); concept of metapopulation -demes and dispersal, intergenic extinctions, age structured populations.
D. Species Interactions: Types of interactions, interspecific competition, herbivory, carnivory, pollination, symbiosis.
E. Community Ecology: Nature of communities; community structure and attributes; levels of species diversity and its measurement; edges and ecotones.
F. Ecological Succession: Types; mechanisms; changes involved in succession; concept of climax.
G. Ecosystem Ecology: Ecosystem structure; ecosystem function; energy flow and mineral cycling (C,N,P); primary production and decomposition; structure and function of some Indian ecosystems: terrestrial (forest, grassland) and aquatic (fresh water, marine, estuarine).
H. Biogeography: Major terrestrial biomes; theory of island biogeography; biogeographical zones of India.
I. Applied Ecology: Environmental pollution; global environmental change; biodiversity: status, monitoring and documentation; major drivers of biodiversity change; biodiversity management approaches. Geographic information system & Modeling, Environmental legislation and policy, Environmental impact and risk assessment
J. Conservation Biology: Principles of conservation, major approaches to management, Indian case studies on conservation/management strategy, land and soil conservation and management, green technologies.
UNIT IX: EVOLUTION AND BEHAVIOUR
A. Emergence of evolutionary thoughts Lamarck, Darwin-concepts of variation, adaptation, struggle, fitness and natural selection; Mendelism; Spontaneity of mutations; The evolutionary synthesis.
B. Origin of cells and unicellular evolution: Origin of basic biological molecules; Abiotic synthesis of organic monomers and polymers; Concept of Oparin and Haldane; Experiment of Miller (1953); The first cell; Evolution of prokaryotes; Origin of eukaryotic cells; Evolution of unicellular eukaryotes; Anaerobic metabolism, photosynthesis and aerobic metabolism.
C. Paleontology and Evolutionary History: The evolutionary time scale; Eras, periods and epoch; Major events in the evolutionary time scale; Origins of unicellular and multi cellular organisms; Major groups of plants; Stages in primate evolution.
D. Molecular Evolution: Concepts of neutral evolution, molecular divergence and molecular clocks; Molecular tools in phylogeny, classification and identification; Protein and nucleotide sequence analysis; origin of new genes and proteins; Gene duplication and divergence.
E. The Mechanisms: Population genetics - Populations, Gene pool, Gene frequency; Hardy-Weinberg Law; concepts and rate of change in gene frequency through natural selection, migration and random genetic drift; Adaptive radiation; Isolating mechanisms; Speciation; Allopatric and Sympatric; Convergent evolution; Sexual selection; Co-evolution.
UNIT X: APPLIED BIOLOGY & METHODS IN PLANT SCIENCE
A. Microbial fermentation and production of Primary & Secondary metabolites.
Tissue and cell culture methods for plants.
Transgenic plants, molecular approaches to diagnosis and strain identification.
Genomics and its application in Plants.
Bioresource and uses of biodiversity.
Breeding in plants including marker selection. assisted
Bioremediation and phytoremediation
Biosensors
B. Molecular Biology and Recombinant DNA methods:
Isolation and purification of RNA, DNA (genomic and plasmid) and proteins, different separation methods.
Analysis of RNA, DNA and proteins by one- and two-dimensional gel electrophoresis, Isoelectric focusing gels. Molecular cloning of DNA or RNA fragments in bacterial and eukaryotic systems. Expression of recombinant proteins using bacterial, animal and plant vectors.
Isolation of specific nucleic acid sequences
Generation of genomic and cDNA libraries in plasmid, phage, cossid, BAC and YAC vectors.
In vitro mutagenesis and deletion techniques, gene knock out in bacterial and eukaryotic organisms. Protein sequencing methods, detection of post translation modification of proteins.
DNA sequencing methods, strategies for genome sequencing.
Methods for analysis of gene expression at RNA and protein level, large scale expression, such as micro array based techniques
Isolation, separation and analysis of carbohydrate and lipid molecules RFLP, RAPD and AFLP techniques
C. Biophysical Method: Molecular analysis using UV/visible, fluorescence, circular dichroism, NMR and ESR spectroscopy Molecular structure determination using X-ray diffraction and NMR, Molecular analysis using light scattering, different types of mass spectrometry and surface plasma resonance methods.
D. Statisitcal Methods: Measures of central tendency and dispersal; probability distributions (Binomial, Poisson and normal); Sampling distribution; Difference between parametric and non-parametric statistics; Confidence Interval; Errors; Levels of significance; Regression and Correlation; t-test; Analysis of variance; X² test;; Basic introduction to Multivariate statistics, etc.
E. Radiolabeling techniques: Detection and measurement of different types of radioisotopes normally used in biology, incorporation of radioisotopes in biological tissues and cells, molecular imaging of radioactive material, safety guidelines. F. Microscopic techniques: Visualizations of cells and subcellular components by light microscopy, resolving powers of different microscopes, microscopy of living cells, scanning and transmission microscopes, different fixation and staining techniques for EM, freeze-etch and freezefracture methods for EM, image processing methods in microscopy.
G. Methods in field biology: Methods of estimating population density of plants, ranging patterns through direct, indirect and remote observations, sampling methods in the study of behavior, habitat characterization: ground and remote sensing methods,
UPPSC Assistant Professor Syllabus 2026 - Overview
| Organization Name | Uttar Pradesh Public Service Commission (UPPSC) |
| Post Name | Assistant Professor (Botany) |
| No. of Posts | 79 |
| Mode of Application | Online |
| Category | Syllabus and Exam Pattern |
| Selection Process | Preliminary Examination, Mains Exam, Interview |
| Job Location | Uttar Pradesh |
| Official Website | uppsc.up.nic.in |
UPPSC Assistant Professor (Botany) Syllabus 2026
General Studies :
1. General Science.
2. Current Events of National and International Importance.
3. History of India (Including Indian National Movement).
4. Indian Polity and Economy.
5. Geography- Indian and world.
6. Mental ability and Statistical data analysis.
Candidates are expected to have general awareness about the above topics with special reference to Uttar Pradesh.
Optional Subject (Botany) :
UNIT I: MOLECULES AND THEIR INTERACTION RELEVANT TO BIOLOGY
A. Structure of atoms, molecules and chemical bonds.
B. Composition, structure and function of biomolecules (carbohydrates, lipids, proteins, nucleic acids and vitrains).
C. Stabilizing interactions (Van der Waals, electrostatic, hydrogen bonding, hydrophobic interaction, etc.).
D. Principles of biophysical chemistry (pH, buffer, reaction kinetics, thermodynamics, colligative properties).
E. Bioenergetics, glycolysis, oxidative phosphorylation, coupled reaction, group transfer, biological energy transducers.
F. Principles of catalysis, enzymes and enzyme kinetics, enzyme regulation, mechanism of enzyme catalysis, isozymes
G. Conformation of proteins (Ramachandran plot, secondary structure, domains, and folds).
H. Conformation of nucleic acids (helix (A, B, Z), t-RNA, micro-RNA).
I. Stability of proteins and nucleic acids.
J. Metabolism of carbohydrates, lipids, amino acids nucleotides and vitamins.
UNIT II: CELLULAR ORGANIZATION
A. Membrane structure and function motif (Structure of model membrane, lipid bilayer and membrane protein diffusion, osmosis, ion channels, active transport, membrane pumps, mechanism of sorting and regulation of intracellular transport, electrical properties of membranes).
B. Structural organization and function of intracellular organelles (Cell wall, nucleus, mitochondria, Golgi bodies, lysosomes, endoplasmic reticulum, peroxisomes, plastids, vacuoles, chloroplast, structure and function of cytoskeleton and its role in motility).
C. Organization of genes and chromosomes (Operon, unique and repetitive DNA, interrupted genes, gene families, structure of chromatin and chromosomes, heterochromatin, euchromatin, transposons).
D. Cell division and cell cycle (Mitosis and meiosis, their regulation, steps in cell cycle, regulation and control of cell cycle).
E Microbial Physiology (Growth yield and characteristics strategies of cell division, stress response)
UNIT III: FUNDAMENTAL PROCESSES
A. DNA replication, repair and recombination (Unit of replication, enzymes involved, replication origin and replication fork, fidelity of replication, extrachromosomal replicons, DNA damage and repair mechanisms, homologous and site-specific recombination).
B. RNA synthesis and processing (transcription factors and machinery, formation of initiation complex, transcription activator and repressor, RNA polymerases capping, elongation, and termination, RNA processing, RNA editing, splicing, and polyadenylation, structure and function of different types of RNA, RNA transport).
C. Protein synthesis and processing (Ribosome, formation of initiation complex, initiation factors and their regulation, elongation and elongation factors, termination, genetic code, aminoacylation of tRNA, tRNA-identity, aminoacyl tRNA synthetase, and translational proof-reading, translational inhibitors, post-translational modification of proteins).
D. Control of gene expression at transcription and translation level (regulating the expression of phages, viruses, prokaryotic and eukaryotic genes, role of chromatin in gene expression and gene silencing).
UNIT IV: CELL COMMUNICATION AND CELL SIGNALING
A. Host parasite interaction- Recognition and entry w processes of different pathogens like bacteria, viruses into re plant host cells, alteration of host cell behavior by C pathogens, virus-induced cell transformation, pathogen-po induced diseases in plants, cell-cell fusion in both normal st and abnormal cells.
B. Cell signaling Hormones and their receptors, cell set surface receptor, signaling through G-protein coupled D receptors, signal transduction pathways, second co messengers, regulation of signaling pathways, bacterial E. and plant two component systems, light signaling in plants, st bacterial chemotaxis and quorum sensing.
C. Cellular communication - Regulation of hematopoiesis, F. general principles of cell communication, cell adhesion and in roles of different adhesion molecules, gap junctions, G. extracellular matrix, integrins, neurotransmission and its fu regulation.
UNIT V: PLANT PHYSIOLOGY
A. Photosynthesis Light harvesting complexes mechanisms of electron transport; photoprotective mechanisms; CO, fixation-C3, C4 and CAM pathways.
B. Respiration and photorespiration - Citric acid cycle; plant mitochondrial electron transport and ATP synthesis-alternate oxidase; photorespiratory pathway.
C. Nitrogen metabolism Nitrate and ammonium assimilation; amino acid biosynthesis.
D. Plant hormones - Biosynthesis, storage, breakdown and transport; physiological effects and mechanisms of action.
E. Sensory photobiology - Structure, function and mechanisms of action of phytochromes, cryptochromes and phytotropins; stomatal movement; photoperiodism and biological clocks.
F. Solute transport and photo assimilate translocation uptake, transport and translocation of water, ions, solutes and macromolecules from soil, through cells, across membranes, through xylem and phloem; transpiration; mechanisms of loading and unloading of photo assimilates.
G. Secondary metabolites Biosynthesis of terpenes, phenols and nitrogenous compounds and their roles.
H. Stress physiology Responses of plants to biotic (pathogen and insects) and abiotic (water, temperature and salt) stresses.
UNIT VI: INHERITANCE BIOLOGY
A. Mendelian principles: Dominance, segregation, independent assortment.
B. Concept of gene: Allele, multiple alleles, pseudo allele, complementation tests
C. Extensions of Mendelian principles: Codominance, incomplete dominance, gene interactions, pleiotropy, genomic imprinting, penetrance and expressivity, phenocopy, linkage and crossing over, sex linkage, sex limited and sex influenced characters.
D. Gene mapping methods: Linkage maps, tetrad analysis, mapping with molecular markers, mapping by using somatic cell hybrids, development of mapping population in plants
E. Extra chromosomal inheritance: Inheritance of Mitochondrial and chloroplast genes, maternal inheritance.
F. Microbial genetics: Methods of genetic transfers -transformation, conjugation, transduction and sex-duction, mapping genes by interrupted mating, fine structure analysis of genes.
G. Quantitative genetics: Polygenic inheritance, heritability and its measurements, QTL mapping
H. Mutation: Types, causes and detection, mutant types -lethal, conditional, biochemical, loss of function, gain of function, germinal verses somatic mutants, insertional mutagenesis
I. Structural and numerical alterations of chromosomes: Deletion, duplication, inversion, translocation, ploidy and their genetic implications.
J. Recombination: Homologous and non-homologous recombination including transposition.
UNIT VII: DIVERSITY OF LIFE FORMS
A. Principles & methods of taxonomy: Concepts of species and hierarchical taxa, biological nomenclature, classical & quantitative methods of taxonomy of plants, animals and microorganisms. Levels of structural organization: Unicellular, colonial and multicellular forms.
B. Levels of organization of tissues, organs & systems. Comparative anatomy, adaptive radiation, adaptive modifications.
C. Outline classification of plants & microorganisms: Important criteria used for classification in each taxon. Classification of plants, animals and microorganisms.
D. Natural history of Indian subcontinent: Major habitat types of the subcontinent, geographic origins and migrations of species.
E. Organisms of health & agricultural importance: Common parasites and pathogens of crops.
F. Organisms of conservation concern: Rare, endangered species. Conservation strategies.
G. Biology and Biodiversity of Viruses, Bacteria, Fungi, Bryophytes, Pteridophytes, Gymnosperms and Angiosperms.
UNIT VIII: ECOLOGICAL PRINCIPLES
A. The Environment: Physical environment; biotic environment; biotic and abiotic interactions.
B. Habitat and Niche: Concept of habitat and niche; niche width and overlap; fundamental and realized niche; resource partitioning; character displacement.
C. Population Ecology: Characteristics of a population; population growth curves; population regulation; life history strategies (rand K selection); concept of metapopulation -demes and dispersal, intergenic extinctions, age structured populations.
D. Species Interactions: Types of interactions, interspecific competition, herbivory, carnivory, pollination, symbiosis.
E. Community Ecology: Nature of communities; community structure and attributes; levels of species diversity and its measurement; edges and ecotones.
F. Ecological Succession: Types; mechanisms; changes involved in succession; concept of climax.
G. Ecosystem Ecology: Ecosystem structure; ecosystem function; energy flow and mineral cycling (C,N,P); primary production and decomposition; structure and function of some Indian ecosystems: terrestrial (forest, grassland) and aquatic (fresh water, marine, estuarine).
H. Biogeography: Major terrestrial biomes; theory of island biogeography; biogeographical zones of India.
I. Applied Ecology: Environmental pollution; global environmental change; biodiversity: status, monitoring and documentation; major drivers of biodiversity change; biodiversity management approaches. Geographic information system & Modeling, Environmental legislation and policy, Environmental impact and risk assessment
J. Conservation Biology: Principles of conservation, major approaches to management, Indian case studies on conservation/management strategy, land and soil conservation and management, green technologies.
UNIT IX: EVOLUTION AND BEHAVIOUR
A. Emergence of evolutionary thoughts Lamarck, Darwin-concepts of variation, adaptation, struggle, fitness and natural selection; Mendelism; Spontaneity of mutations; The evolutionary synthesis.
B. Origin of cells and unicellular evolution: Origin of basic biological molecules; Abiotic synthesis of organic monomers and polymers; Concept of Oparin and Haldane; Experiment of Miller (1953); The first cell; Evolution of prokaryotes; Origin of eukaryotic cells; Evolution of unicellular eukaryotes; Anaerobic metabolism, photosynthesis and aerobic metabolism.
C. Paleontology and Evolutionary History: The evolutionary time scale; Eras, periods and epoch; Major events in the evolutionary time scale; Origins of unicellular and multi cellular organisms; Major groups of plants; Stages in primate evolution.
D. Molecular Evolution: Concepts of neutral evolution, molecular divergence and molecular clocks; Molecular tools in phylogeny, classification and identification; Protein and nucleotide sequence analysis; origin of new genes and proteins; Gene duplication and divergence.
E. The Mechanisms: Population genetics - Populations, Gene pool, Gene frequency; Hardy-Weinberg Law; concepts and rate of change in gene frequency through natural selection, migration and random genetic drift; Adaptive radiation; Isolating mechanisms; Speciation; Allopatric and Sympatric; Convergent evolution; Sexual selection; Co-evolution.
UNIT X: APPLIED BIOLOGY & METHODS IN PLANT SCIENCE
A. Microbial fermentation and production of Primary & Secondary metabolites.
Tissue and cell culture methods for plants.
Transgenic plants, molecular approaches to diagnosis and strain identification.
Genomics and its application in Plants.
Bioresource and uses of biodiversity.
Breeding in plants including marker selection. assisted
Bioremediation and phytoremediation
Biosensors
B. Molecular Biology and Recombinant DNA methods:
Isolation and purification of RNA, DNA (genomic and plasmid) and proteins, different separation methods.
Analysis of RNA, DNA and proteins by one- and two-dimensional gel electrophoresis, Isoelectric focusing gels. Molecular cloning of DNA or RNA fragments in bacterial and eukaryotic systems. Expression of recombinant proteins using bacterial, animal and plant vectors.
Isolation of specific nucleic acid sequences
Generation of genomic and cDNA libraries in plasmid, phage, cossid, BAC and YAC vectors.
In vitro mutagenesis and deletion techniques, gene knock out in bacterial and eukaryotic organisms. Protein sequencing methods, detection of post translation modification of proteins.
DNA sequencing methods, strategies for genome sequencing.
Methods for analysis of gene expression at RNA and protein level, large scale expression, such as micro array based techniques
Isolation, separation and analysis of carbohydrate and lipid molecules RFLP, RAPD and AFLP techniques
C. Biophysical Method: Molecular analysis using UV/visible, fluorescence, circular dichroism, NMR and ESR spectroscopy Molecular structure determination using X-ray diffraction and NMR, Molecular analysis using light scattering, different types of mass spectrometry and surface plasma resonance methods.
D. Statisitcal Methods: Measures of central tendency and dispersal; probability distributions (Binomial, Poisson and normal); Sampling distribution; Difference between parametric and non-parametric statistics; Confidence Interval; Errors; Levels of significance; Regression and Correlation; t-test; Analysis of variance; X² test;; Basic introduction to Multivariate statistics, etc.
E. Radiolabeling techniques: Detection and measurement of different types of radioisotopes normally used in biology, incorporation of radioisotopes in biological tissues and cells, molecular imaging of radioactive material, safety guidelines. F. Microscopic techniques: Visualizations of cells and subcellular components by light microscopy, resolving powers of different microscopes, microscopy of living cells, scanning and transmission microscopes, different fixation and staining techniques for EM, freeze-etch and freezefracture methods for EM, image processing methods in microscopy.
G. Methods in field biology: Methods of estimating population density of plants, ranging patterns through direct, indirect and remote observations, sampling methods in the study of behavior, habitat characterization: ground and remote sensing methods,
UPPSC Assistant Professor (Botany) Exam Pattern 2026
Preliminary Examination
Duration : 120 Minutes
Negative Marking : 0.33
| S.No | Subject | No.of uestion | Marks |
| 1 | General Studies | 30 | 60 |
| 2 | Optional Subject | 90 | 90 |
| Total | 120 | 150 |
Mains Examination
Duration : 180 Minutes
| S.No | Subject | No.of Question | Marks |
| 1 | Optional Subject | (10+10) 20 Section A: 10 Short Answer Questions (125 words each) Section B: 10 Long Answer Questions (200 words each) |
(80+120) 200 Section A: 10 questions x 8 marks = 80 marks Section B: 10 questions x 12 marks = 120 marks |
| Total | 120 | 150 |
Main Exam Date 2026 : 15th September 2026
Starting Date of Application Form : 04th September 2025
Last Date of Application Form : 06th October 2025
Total Post : 79
FAQs
Uppsc Assistant Professor (botany) 2026 Exam Syllabus Frequently Asked Questions (FAQ's)
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