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Latest NIB Laboratory Technician Exam Question (Objective Questions), MCQ in English

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Laboratory Technician

Question 1Single Answer
What is the primary purpose of Sabouraud Dextrose Agar in mycology?
  1. A
    To inhibit fungal growth
  2. B
    To culture bacteria
  3. C
    To stain fungal elements
  4. D
    To promote fungal growth
Question 2Single Answer
Which stain is commonly used to visualize fungal elements in a wet mount?
  1. A
    Gram stain
  2. B
    KOH (Potassium Hydroxide)
  3. C
    Ziehl-Neelsen stain
  4. D
    Hematoxylin-Eosin
Question 3Single Answer
What type of specimen is most commonly used for diagnosing dermatophyte infections?
  1. A
    Blood
  2. B
    Skin scrapings
  3. C
    Urine
  4. D
    Stool
Question 4Single Answer
Which fungus is typically associated with thrush in immunocompromised patients?
  1. A
    Candida albicans
  2. B
    Aspergillus fumigatus
  3. C
    Cryptococcus neoformans
  4. D
    Rhizopus oryzae
Question 5Single Answer
What is the role of Calcofluor White in mycology?
  1. A
    It inhibits fungal growth
  2. B
    It measures antifungal susceptibility
  3. C
    It differentiates bacterial species
  4. D
    It enhances fungal fluorescence under UV light
Question 6Single Answer
Which medium is preferred for culturing dimorphic fungi?
  1. A
    Blood agar
  2. B
    Brain Heart Infusion agar
  3. C
    Sabouraud Dextrose Agar
  4. D
    MacConkey agar
Question 7Single Answer
What is the purpose of a biosafety cabinet in a mycology lab?
  1. A
    To prevent contamination and protect the technician
  2. B
    To store fungal cultures
  3. C
    To incubate fungal cultures
  4. D
    To perform DNA extraction
Question 8Single Answer
Which test is used to identify Cryptococcus neoformans in cerebrospinal fluid?
  1. A
    Urease test
  2. B
    India ink preparation
  3. C
    Carbohydrate assimilation test
  4. D
    KOH mount
Question 9Single Answer
What is the typical incubation temperature for fungal cultures?
  1. A
    4°C
  2. B
    45°C
  3. C
    37°C
  4. D
    25–30°C
Question 10Single Answer
Which fungal structure is primarily observed during microscopic examination?
  1. A
    Nucleus
  2. B
    Hyphae
  3. C
    Mitochondria
  4. D
    Cell membrane
Question 11Single Answer
What is the primary goal of antifungal susceptibility testing?
  1. A
    To identify fungal species
  2. B
    To determine effective antifungal drugs
  3. C
    To culture bacteria
  4. D
    To stain fungal elements
Question 12Single Answer
Which fungus is commonly associated with pulmonary infections in immunocompromised patients?
  1. A
    Trichophyton rubrum
  2. B
    Microsporum canis
  3. C
    Candida tropicalis
  4. D
    Aspergillus fumigatus
Question 13Single Answer
What is the purpose of the urease test in mycology?
  1. A
    To detect bacterial contamination
  2. B
    To measure fungal growth rate
  3. C
    To differentiate fungal species
  4. D
    To stain fungal spores
Question 14Single Answer
Which sample is least likely to be used for mycological analysis?
  1. A
    Nail clippings
  2. B
    Saliva
  3. C
    Blood
  4. D
    Sputum
Question 15Single Answer
What is the function of potato dextrose agar in mycology?
  1. A
    To promote sporulation in fungi
  2. B
    To inhibit fungal growth
  3. C
    To culture viruses
  4. D
    To perform biochemical tests
Question 16Single Answer
Which biosafety level is typically required for handling pathogenic fungi like Histoplasma?
  1. A
    BSL-1
  2. B
    BSL-2
  3. C
    BSL-3
  4. D
    BSL-4
Question 17Single Answer
What is the primary source of contamination in fungal cultures?
  1. A
    Environmental molds
  2. B
    Bacterial spores
  3. C
    Viral particles
  4. D
    Chemical residues
Question 18Single Answer
Which fungal species is commonly identified using a germ tube test?
  1. A
    Aspergillus niger
  2. B
    Fusarium solani
  3. C
    Rhizopus oryzae
  4. D
    Candida albicans
Question 19Single Answer
What is the purpose of a wet mount in mycology?
  1. A
    To culture fungi
  2. B
    To perform DNA sequencing
  3. C
    To observe fungal structures microscopically
  4. D
    To measure antifungal resistance
Question 20Single Answer
Which antifungal drug is commonly tested in susceptibility assays?
  1. A
    Penicillin
  2. B
    Streptomycin
  3. C
    Tetracycline
  4. D
    Fluconazole
Question 21Single Answer
What is the role of a laboratory information system (LIS) in mycology?
  1. A
    To document procedures and results
  2. B
    To culture fungi
  3. C
    To stain fungal specimens
  4. D
    To calibrate microscopes
Question 22Single Answer
Which fungal infection is commonly diagnosed using skin scrapings?
  1. A
    Cryptococcosis
  2. B
    Aspergillosis
  3. C
    Tinea corporis
  4. D
    Histoplasmosis
Question 23Single Answer
What is the primary use of chloramphenicol in fungal culture media?
  1. A
    To promote fungal growth
  2. B
    To enhance staining
  3. C
    To inhibit bacterial growth
  4. D
    To increase sporulation
Question 24Single Answer
Which technique is used for definitive fungal identification?
  1. A
    Gram staining
  2. B
    Blood culture
  3. C
    PCR and sequencing
  4. D
    Wet mount
Question 25Single Answer
What is the typical appearance of Aspergillus colonies on culture media?
  1. A
    Velvety with powdery spores
  2. B
    Smooth and creamy
  3. C
    Waxy and translucent
  4. D
    Shiny and moist
Question 26Single Answer
Which specimen is used to diagnose fungal meningitis?
  1. A
    Cerebrospinal fluid (CSF)
  2. B
    Blood
  3. C
    Urine
  4. D
    Stool
Question 27Single Answer
What is the purpose of cycloheximide in fungal culture media?
  1. A
    To inhibit saprophytic fungi
  2. B
    To promote yeast growth
  3. C
    To enhance bacterial growth
  4. D
    To stain fungal elements
Question 28Single Answer
Which fungal structure is observed in a KOH preparation of dermatophytes?
  1. A
    Budding yeast
  2. B
    Septate hyphae
  3. C
    Capsule
  4. D
    Endospores
Question 29Single Answer
What is the primary function of an incubator in a mycology lab?
  1. A
    To maintain optimal temperature for fungal growth
  2. B
    To sterilize media
  3. C
    To perform DNA extraction
  4. D
    To store specimens
Question 30Single Answer
Which fungus is associated with a "spaghetti and meatball" appearance in KOH preparations?
  1. A
    Trichophyton mentagrophytes
  2. B
    Aspergillus flavus
  3. C
    Candida glabrata
  4. D
    Malassezia furfur
Question 31Single Answer
What is the purpose of quality control in a mycology lab?
  1. A
    To ensure accurate and reliable test results
  2. B
    To culture bacteria
  3. C
    To stain fungal specimens
  4. D
    To calibrate microscopes
Question 32Single Answer
Which test differentiates Candida species from other yeasts?
  1. A
    Urease test
  2. B
    Germ tube test
  3. C
    India ink preparation
  4. D
    KOH mount
Question 33Single Answer
What is the typical growth time for most fungal cultures?
  1. A
    24 hours
  2. B
    1–4 weeks
  3. C
    48–72 hours
  4. D
    6–8 weeks
Question 34Single Answer
Which fungus is commonly associated with bird droppings?
  1. A
    Cryptococcus neoformans
  2. B
    Candida albicans
  3. C
    Aspergillus niger
  4. D
    Rhizopus oryzae
Question 35Single Answer
What is the primary purpose of a tease mount in mycology?
  1. A
    To culture fungi
  2. B
    To measure antifungal susceptibility
  3. C
    To perform biochemical tests
  4. D
    To observe fungal morphology
Question 36Single Answer
Which antifungal is commonly used to treat Candida infections?
  1. A
    Amphotericin B
  2. B
    Penicillin
  3. C
    Ciprofloxacin
  4. D
    Azithromycin
Question 37Single Answer
What is the role of a laminar flow hood in a mycology lab?
  1. A
    To store fungal cultures
  2. B
    To perform DNA sequencing
  3. C
    To incubate fungal cultures
  4. D
    To provide a sterile working environment
Question 38Single Answer
Which fungal infection is commonly diagnosed using sputum samples?
  1. A
    Tinea pedis
  2. B
    Aspergillosis
  3. C
    Candidiasis
  4. D
    Sporotrichosis
Question 39Single Answer
What is the primary advantage of using molecular methods in fungal identification?
  1. A
    Faster culture growth
  2. B
    Lower cost than microscopy
  3. C
    High specificity and accuracy
  4. D
    Simpler sample preparation
Question 40Single Answer
Which fungal species is associated with rose gardener’s disease?
  1. A
    Aspergillus fumigatus
  2. B
    Candida albicans
  3. C
    Sporothrix schenckii
  4. D
    Cryptococcus gattii
Question 41Single Answer
What is the purpose of carbohydrate assimilation tests in mycology?
  1. A
    To identify yeast species
  2. B
    To stain fungal elements
  3. C
    To culture molds
  4. D
    To measure antifungal resistance
Question 42Single Answer
Which fungus is commonly identified by its "spider web" colony morphology?
  1. A
    Fusarium solani
  2. B
    Aspergillus niger
  3. C
    Rhizopus oryzae
  4. D
    Penicillium marneffei
Question 43Single Answer
What is the primary biosafety concern when handling fungal cultures?
  1. A
    Aerosolized spores
  2. B
    Chemical spills
  3. C
    Electrical hazards
  4. D
    Radiation exposure
Question 44Single Answer
Which stain is used to detect Pneumocystis jirovecii in respiratory specimens?
  1. A
    KOH
  2. B
    Giemsa stain
  3. C
    Gram stain
  4. D
    Ziehl-Neelsen stain
Question 45Single Answer
What is the purpose of a negative control in antifungal susceptibility testing?
  1. A
    To ensure test reliability
  2. B
    To promote fungal growth
  3. C
    To stain fungal elements
  4. D
    To culture bacteria
Question 46Single Answer
Which fungal infection is associated with "black mold"?
  1. A
    Aspergillosis
  2. B
    Candidiasis
  3. C
    Mucormycosis
  4. D
    Histoplasmosis
Question 47Single Answer
What is the primary use of lactophenol cotton blue in mycology?
  1. A
    To culture fungi
  2. B
    To stain fungal structures for microscopy
  3. C
    To inhibit bacterial growth
  4. D
    To perform DNA extraction
Question 48Single Answer
Which fungus is commonly associated with systemic infections in AIDS patients?
  1. A
    Trichophyton rubrum
  2. B
    Microsporum canis
  3. C
    Aspergillus flavus
  4. D
    Histoplasma capsulatum
Question 49Single Answer
What is the purpose of a positive control in mycology quality control?
  1. A
    To inhibit fungal growth
  2. B
    To verify test accuracy
  3. C
    To stain fungal specimens
  4. D
    To culture bacteria
Question 50Single Answer
Which fungal structure is characteristic of dermatophytes?
  1. A
    Arthroconidia
  2. B
    Budding yeast
  3. C
    Capsule
  4. D
    Endospores
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